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| Registros recuperados : 13 | |
| 1. |  | LIMA, S. C. G. de; LOURENÇO JUNIOR, J. de B.; GARCIA, A. R.; NAHUM, B. de S.; SILVA, A. G. M. e; RODRIGUES, L. S.; MENEZES, B. P. de; NERES, L. de S. Características físico-químicas e microbiológicas do leite de búfalas suplementadas com resíduos da agroindústria, na Amazônia Oriental. Revista Higiene Alimentar, v. 25, n. 194/195, p. 319-320, mar./abr. 2011. Encarte. V Congresso Latinoamericano e XI Congresso Brasleiro de Higienistas de Alimentos. O alimento nas próximas décadas: produzir sem agredir: anais. Salvador, 2011.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | MENEZES, B. P. de; LOURENÇO JUNIOR, J. de B.; SILVA, A. G. M. e; RODRIGUES, L. S.; GARCIA, A. R.; NAHUM, B. de S. Consumo voluntário de subproduto da agroindústria cosmetica por ovinos na Amazônia Oriental. In: CONGRESSO LATINO AMERICANO DE NUTRIÇÃO ANIMAL, 4., 2010, São Pedro, SP. Anais... [Campinas]: CBNA, 2010. p. 291-292.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | RODRIGUES, L. S.; LOURENÇO JUNIOR, J. de B.; SILVA, A. G. M. e; MENEZES, B. P. de; GARCIA, A. R.; NAHUM, B. de S. Consumo voluntário de torta de cupuaçu (Theobroma grandiflorum), por ovinos, na Amazônia Oriental. In: CONGRESSO LATINO AMERICANO DE NUTRIÇÃO ANIMAL, 4., 2010, São Pedro, SP. Anais... [Campinas]: CBNA, 2010. p. 283-284.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | MENEZES, B. P. de; LOURENÇO JUNIOR, J. de B.; SILVA, A. G. M. e; RODRIGUES, L. S.; GARCIA, A. R.; NAHUM, B. de S. Digestibilidade aparente da matéria seca e matéria orgânica da torta de murmuru (Astrocaryum murmuru vr. murmuru Mart.) em ovinos. Veterinária e Zootecnia, v. 18, n. 4, p. 1229-1231, dez. 2011. supl. 3.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | BARBOSA, N. G. S.; RODRIGUEZ, N. M.; FERNANDES, P. C. C.; GARCIA, A. R.; NAHUM, B. de S.; SALIBA, E. S.; BORGES, I.; ÁVILA, S. C.; MENEZES, B. P.; RIBEIRO, O. A. V.; OLIVEIRA, M. E. C.; QUINZEIRO NETO, T. Intake and digestibility of river buffalo steers (Bubalus bubalis) fed different levels of palm kernel cake: effect of diet neutral detergent fiber, digestible energy, crude protein and extract ether. Revista Veterinaria, v. 21, supl. 1, p. 146-150, 2010. Proceeding of World Buffalo Congress, 9., 2010, Buenos Aires.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | MENEZES, B. P. de; RODRIGUES, L. S.; LOURENÇO JUNIOR, J. de B.; SILVA, A. G. M. e; ANDRADE, S. J. T. de; SILVA, J. A. R. da; FATURI, C.; GARCIA, A. R.; NAHUM, B. de S.; BARBOSA, A. V. C.; BUDEL, J. C. de C.; ARAUJO, G. S. Intake, digestibility, and nitrogen balance of rations containing different levels of murumuru meal in sheep diets. Semina: Ciências Agrárias, Londrina, v. 37, n. 1, p. 415-428, jan./fev. 2016.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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| 8. | | RODRIGUES, L. S.; MENEZES, B. P. de; SILVA, A. G. M. e; FATURI, C.; SILVA, J. A. R. da; GARCIA, A. R.; NAHUM, B. de S.; ANDRADE, S. J. T. de; LOURENÇO JUNIOR, J. de B. Ovine feed intake, digestibility, and nitrogen balance in feeds containing different amounts of cupuaçu meal. Semina: Ciências Agrárias, Londrina, v. 36, n. 4, p. 2799-2808, 2015.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
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| 9. | | BARBOSA, N. G. S.; RODRIGUEZ, N. M.; BARBOSA, J. D.; OLIVEIRA, C. M. C.; FERNANDES, P. C. C.; ÁVILA, S. C.; SALIBA, E. S.; BORGES, I.; QUINZEIRO NETO, T.; MENEZES, B. P. Rumen protozoa population in river steers buffalo (Bbubalus bubalis) fed different levels of palm kernel cake. Revista Veterinaria, v. 21, supl. 1, p. 697-699, 2010. Proceeding of World Buffalo Congress, 9., 2010, Buenos Aires.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | MENEZES, B. P. de; RODRIGUES, L. S.; LOURENÇO JUNIOR, J. de B.; SILVA, A. G. M. e; ANDRADE, S. J. T. de; GARCIA, A. R. Subproduto do murumuru (Astrocaryum murumuru vr. murumuru Mart.) na alimentação de ruminantes na Amazônia Oriental. In: SIMPÓSIO DE PESQUISA INTERDISCIPLINAR DA AMAZÔNIA ORIENTAL, 1., 2011, Belém, PA. Diálogos interdisciplinares em busca da integração regional: anais. Belém, PA: UEPA, 2011.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 11. | | LIMA, S. C. G. de; LOURENÇO JUNIOR, J. de B.; GARCIA, A. R.; NAHUM, B. de S.; SILVA, A. G. M. e; GUIMARÃES, C. M. C.; RODRIGUES, L. S.; MENEZES, B. P. de. Suplementação de búfalas com resíduos agroindustriais: efeito na qualidade sensorial e físico-química do leite. In: CONGRESSO LATINO AMERICANO DE NUTRIÇÃO ANIMAL, 4., 2010, São Pedro, SP. Anais... [Campinas]: CBNA, 2010. p. 239-240.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | BARBOSA, N. G. S.; RODRIGUEZ, N. M.; FERNANDES, P. C. C.; RIBEIRO, O. A. V. R.; MENEZES, B. P.; GARCIA, A. R. Torta de amêndoa de dendê em dietas para bubalinos: metabolismo ruminal. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém, PA. O desenvolvimento da produção animal e a responsabilidade frente a novos desafios: anais. Belém, PA: SBZ, 2011.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 13. | | BARBOSA, N. G. S.; FERNANDES, P. C. C.; RODRIGUEZ, N. M.; MENEZES, B. P.; RIBEIRO, O. A. V.; MOREIRA, G. R. Volume ruminal em búfalos suplementados com torta de amêndoa de dendê: método das bolas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 48., 2011, Belém. O Desenvolvimento da produção animal e a responsabilidade frente a novos desafios - anais. Belém: SBZ: UFRA, 2011.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 13 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/05/2017 |
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Data da última atualização: |
29/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BALARO, M. F. A.; FREITAS, V. J. de F.; OLIVEIRA, R. V. de; BRANDÃO, F. Z. |
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Afiliação: |
Carolina Cerqueira Sarmento Olivares; Joanna Maria Gonçalves de Souza-Fabjan; JEFERSON FERREIRA DA FONSECA, CNPC; Mario Felipe Alvarez Balaro; Vicente José de Figueirêdo Freitas; Rodrigo Vasconcelos de Oliveira; Felipe Zandonadi Brandão. |
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Título: |
Comparison of different sperm selection techniques in ram frozen-thawed sperm. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 45, p. 1431, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Resumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique. MenosResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of inte... Mostrar Tudo |
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Palavras-Chave: |
CASA; CTC; Plasma membrane integrity; Protocolo; Reproductive techniques; Sperm preparation. |
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Thesagro: |
Espermatozóide; Ovino; Reprodução animal; Sêmen. |
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Thesaurus NAL: |
Reproduction; Sheep; sperm capacitation; Spermatozoa; Treaties. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160322/1/CNPC-2017-Comparison.pdf
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Marc: |
LEADER 04855naa a2200373 a 4500
001 2070178
005 2017-05-29
008 2017 bl uuuu u00u1 u #d
100 1 $aOLIVARES, C. C. S.
245 $aComparison of different sperm selection techniques in ram frozen-thawed sperm.
260 $c2017
520 $aResumo: Background: The success of fertilization is directly associated with semen quality and the sperm preparation. Considering the common use of cryopreserved spermatozoa, there is a need to develop strategies for sperm preparation in order to achieve a sperm sample of high quality through a rigorous selection of sperm. Thus, sperm cells are being more extensively investigate. This study aimed evaluating the influence of different sperm selection techniques on ram sperm parameters in semen preparation. Materials, Methods & Results: Frozen-thawed commercial semen from 10 Santa Inês rams was subjected to either: swim-up, Percoll, mini-Percoll, sperm washing by centrifugation or a control group. After each technique, samples were incubated at 37°C for 1 h, 2 h and 3 h. At post-selection moment (0 h) and at each interval, sperm recovery rate, motility, capacitation and plasma membrane (PM) integrity were analyzed. The lowest (P < 0.05) recovery rate was recorded after swim-up (1.0 ± 0.3%), whilst the others were similar (P > 0.05). Most part of motility parameters were not affected (P > 0.05) by the technique at 0 h; just swim-up obtained higher (P < 0.05) values for VSL (41.8 ± 11.1) and VAP (46.9 ± 11.2). Overall, swim-up presented higher (P < 0.05) values for most of motility parameters over time of incubation. The control group led to more (P < 0.05) capacitated cells (46.8 ± 2.3%), whilst Percoll, mini-Percoll and swim-up were similar (~33%; P > 0.05), regardless of interval incubation. However, the latter three techniques presented more (P < 0.05) reacted cells. Swim-up recovered a higher (P < 0.05) number of intact cells (32.1 ± 6.4%), and Percoll, mini-Percoll and control group were similar (P > 0.05). Discussion: The present study evaluated the sperm cell and its preparation for receiving the oocyte under optimal conditions. evaluate the sperm cell and its preparation for receiving the oocyte under optimal conditions. Although the swim-up technique promoted higher rates for some of the sperm parameters evaluated, Percoll protocols are the most widely used procedures for selection during the sperm preparation in many species, possibly because of its greater speed, practical method and convenience compared to the swim-up technique. Percoll and mini-Percoll recovered approximately 10 times more cells than swim-up, which is an important feature to be considered during sperm preparation for in vitro fertilization (IVF), being possible to use only one semen straw. The high capacitated and acrosome reaction rates observed after the treatments in the current study, are probably reinforced by changes in sperm cells caused by the cryopreservation process. In order to strength the evidence that frozen-thawed sperm, even after selection, is sensible and reactive to capacitation-like events, we demonstrated the capacitated and acrosome-reacted cell values immediately after the selection treatments behaved differently than when authors used ram fresh sperm. Possibly, this capacitation-like changes observed in frozen-thawed sperm occurs regardless of the selection treatment used. Analyzing the motility parameters immediately after the selection, all treatments maintained or increased the rates compared to the control group. The swim-up, mini-Percoll and Percoll did not differ in any parameters. Given that Percoll and mini-Percoll did not show differences in relation to swim-up for motility parameters, such techniques can be used to replace the latter, obtaining similar sperm samples with good quality. However, swim-up technique involves a procedure that recovers a clean fraction without debris and other types of cells, with high rate of mobile sperm with excellent quality, reason why it can justify the higher recovery of intact spermatozoa after the technique.
650 $aReproduction
650 $aSheep
650 $asperm capacitation
650 $aSpermatozoa
650 $aTreaties
650 $aEspermatozóide
650 $aOvino
650 $aReprodução animal
650 $aSêmen
653 $aCASA
653 $aCTC
653 $aPlasma membrane integrity
653 $aProtocolo
653 $aReproductive techniques
653 $aSperm preparation
700 1 $aSOUZA-FABJAN, J. M. G. de
700 1 $aFONSECA, J. F. da
700 1 $aBALARO, M. F. A.
700 1 $aFREITAS, V. J. de F.
700 1 $aOLIVEIRA, R. V. de
700 1 $aBRANDÃO, F. Z.
773 $tActa Scientiae Veterinariae$gv. 45, p. 1431, 2017.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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